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The normalized average tag counts in 500 bp windows are also highly comparable among aliquots (Table 2, R2 = 0.93 0.97).
(B ) Ranking of TRIM33 occupied sites based on average tag counts obtained from B-ALL ChIP-seq analysis.
To calculate average tag counts from reproducible peaks, tag counts were normalized to total mapped reads, and further ranked by tag counts.
(A ) Ranking of TRIM33 occupied sites based on average tag counts obtained from ChIP-seq analysis in the indicated cell lines.
Furthermore, comparison of normalized average tag counts in 500 bp windows between biological replicates for PolII and Ste12 revealed strong correlations (Table 2, R2 = 0.89 0.97).
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The average tag count for MMP1 was 216 tags and the median 180 tags.
Note that the average tag count calculated here is corrected by a factor which takes into consideration that the real average tag count will be greater once ambiguous tags are included.
We then normalized each experiment by dividing the average tag count for each bin by the total tag count for the experiment.
We then use the numbers of unique tags associated with those sites to calculate the average tag count and standard deviation for each site genome wide.
Tags were filtered for more than 10 fold higher average tag count in nuclear versus cytoplasmic samples from both cell types and mapped to transcripts using BLAT [ 19].
Normalized average tag count for each 500 bp bin were compared between two experiments among barcoded and non-barcoded samples for a particular factor.
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