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The average swim speeds (Fig. 2B) for Atxn2 ko mice and wild type littermates were similar on day 14.
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Calves swimming in echelon showed a 28% increase in average swim speed and a 19% increase in distance per stroke.
The average swim speed (cm/s) and latency (s) to reach the platform were scored on all trials and analyzed.
Path length, average swim speed and time spent resting were analyzed as described for platform latency in Figure 2.
Neither brivaracetam nor ethosuximide treatment affected average swim speed or average resting time across mouse cohorts (B, C, E, F). + Indicates drug therapy; - indicates vehicle.
At 1 week, there was no notable difference in the average swim speed or total distance swum in a 10 minute period (supplementary material Fig. S6B).
Further experiments involving deeper dives (>50 m) may provide further insight into this apparent discrepancy, because initial swim speeds should have less effect on the average swim speed as the distance to the bottom is increased.
Fig. 4A displays the raw average swimming speeds during the whole trial as a function of the genotype of animals under non-aversive circumstances (25°C).
However, comparing the swimming speed of a mutant strain of mice with the raw average swimming speeds of control mice during an entire trial does not allow distinguishing between an intrinsic inability to sustain typical levels of swimming speed because of primary motor dysfunction or behavioral and/or neurosensory artifacts.
No genotype-dependent differences in raw average swimming speeds (Fig. 4A inset) were observed and both Dyrk1A+/− and their controls showed a reduction in swimming speed along the 60 s of the trial (MANOVA, "time interval": F(1 59) = 13.741, P<0.0001; Fig. 4A).
Surprisingly, under the less aversive circumstances (25°C) that were the same used in the spatial learning task (water maze), no genotype-dependent differences in average swimming speeds are observed, contrary to the results attained when the escape platform was present in the pool.
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