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C. meneghiniana and C. ovata had an average sterol content of 7 8 µg mgC−1.
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The highest sterol content (10 µg mgC−1 on average) was found in S. quadricauda.
In C. globosa the lowest sterol content was observed (4 µg mgC−1 on average).
It was found that the key factor controlling nystatin photophysical properties in the ternary lipid mixtures was their ergosterol/cholesterol molar ratio and not their overall sterol content.
Phytosterols were not detectable in the sterol chromatograms for Turbo yeast or MYA-796 grown using GJ + tfosEp (Table 2 and Figure 4), and indeed the sterol content of GJ was negligible; however, we did find residual ryegrass pulp, a by-product of the grass juice extraction process, to be rich in plant sterols (data not shown).
The saponification of free sterol studied by Yan et al. showed that the free sterol content in the feed solution after the saponification increased from 6.64 to 9.30% as the NaOH/SODD mass ratio increased from 0.17/1 to 0.33/1 (w/w) [21].
Total sterol content was extracted by saponification [50].
Sterol content was also evaluated using the quantitative fluorimetric kit "Amplex Red Sterol Assay Kit" (Molecular Probes).
No significant change in sterol content was seen when we examined the sum of sterols in C. ovata.
The species with the lowest sterol content, C. globosa, contained total sterol contents of <5 µg mgC−1, which is clearly below all suggested saturation levels.
Phosphorus-limitation reversed the change of sterol content with light intensity, i.e., sterol content decreased with increasing light at low phosphorus supply.
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