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Overall, 10.8% (3.7/33.8 Mb) of chromosome 22 is duplicated, with an average sequence identity of 95.4%.
The protocol is tested on 52 nonhomologous single-domain proteins, with an average sequence identity of 24% between the designed sequences and the native sequences.
The average sequence identity between the GPCR orthologues in human and S. kowalevskii is around 47%, and this is same as observed in couple of the closest vertebrate relatives, Ciona intestinalis (41%) and Branchiostoma floridae (~ 47%).
Average sequence identity is around 60 70% in each sub-family and 20% between the two.
In addition, the average sequence identity between drug-target pairs sharing the same drugs (i.e. promiscuous drugs) was only 0.0311, showing the diversity of targets in promiscuous drugs and implying the difficulty of clustering drug-target pairs by drug or target information only (Table S8).
An average sequence identity was calculated for the remaining sequences.
The average sequence identity between isolates was 96%.
The sequence with the lowest average sequence identity with respect to all others in a given MSA was removed until the average sequence identity was above 25%.
Interestingly, the average sequence identity increases as the function similarity bin increases, and the average sequence identity in Bin 10 for each category is always relatively high.
The Classic subfamily members from the three genomes share an average sequence identity of 15.5%, while the PlusC OBPs share 17.3% average sequence identity.
The combined average sequence identity (referred to as %id) of all protein families was 91.61%.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com