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Briefly, duplicate luminometer readings were taken from each sample over 20 s intervals, and the average relative light unit readings were used to determine ATP content in the samples against the standard curve.
Presented are average relative light units and the S.E.
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The average relative fluorescence light intensity (Iav(7)) did not significantly change during the study.
> -wrap-foot> Vareexpressedpressed as mean ± standard deviation AUC area under the curve, I av (7) average relative fluorescence light intensity over the first 7 min * p < 0.05 compared to baseline The number of capillaries did not change significantly during the study period.
Average luminescence (in relative light units; RLU) was divided by the mean RFU obtained for the same well from using the CellTiter-Fluor kit (Promega).
The ATP content of each sample was calculated as the average of the relative light readings and based on the ATP standard curve.
For the QTL analysis, plants were grown in a greenhouse (average air temperature, 30°C; average relative humidity, 50%; natural lighting).
RDR was evaluated quantitatively in the IK-CSSLs and KD-F2 based on a basket method that uses open stainless-steel-mesh baskets filled with soil in a greenhouse (average air temperature, 30°C; average relative humidity, 50%; natural lighting), as described previously (Uga et al. 2011).
Study animals (pair-housed until day of exposure and testing) were kept in a climate-controlled room at 25°C and 45 60% average relative humidity and a 12-hr light cycle (lights on from 1200 hours to 2400 hours).
Thus, the average relative hyperpolarization of 22 mV caused by light-stimulation of Arch was stable for at least that long, or even more pronounced before the time spent at ambient conditions.
Timed pregnant Long Evans rats (n = 10; Charles River, NC, USA) were individually housed in polysulfone caging within a humidity- and temperature-controlled room with a 14-h light, 10-h dark cycle (lights on from 7 00 to 21 00) at 23°C and 50% average relative humidity at the Biological Resource Facility at North Carolina State University (NCSU).
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