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An average reading from both arms was calculated for analysis.
A. Subtract the average reading from media-only wells from the wells with cells.
The mean PI is calculated as the average reading from each side combined.
2. The next day after seeding, read GFP fluorescence (Synergy HT Microplate Reader). A. Subtract the average reading from media-only wells from the wells with cells.
Data analysis: A. Remove background fluorescence by subtracting the average reading from media-only wells from the wells with cells for each plate reading.
On day 1 after seeding, read GFP fluorescence (Synergy HT Microplate Reader). A. Subtract the average reading from media-only wells from the wells with cells.
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