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The informative Class 2 SNPs by experiments are defined as those SNP-tagged loci that have more than 20% decrease in the average probe intensity ratios.
In cases were multiple probes mapped to the same Entrez ID, the average probe intensity was calculated.
Then, the average probe intensity from the smoothed L2-poly(A) data was assigned to that gene.
To do so, we used a Bayesian clustering approach to infer outlying individuals on the basis of call rate, heterozygosity, ancestry, and average probe intensity.
The average probe intensity (average of antilogs of the raw data) from the normalized data for all replicates of each parasite isolate was calculated.
The methylation status for each individual CpG locus was calculated as the ratio of fluorescent signals: β = max(M,0)/[max(M,0) + max(U,0) + 100], using the average probe intensity for the methylated (M) and unmethylated (U) alleles.
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Log2 ratios of the average probe intensities between the experimental and known genome were calculated and input into CGHScan using a significance value of 0.05.
Average probe intensities for the TGF-β receptors I and II as well as TGF β ligands 1 and 3 were also elevated in the MSL subtype in comparison to the rest of the TNBC subtypes (Additional file 2: Figure S1).
Consequentially, this test-statistic was utilized as it avoids introducing unnecessary variance by averaging probe intensity data from probes with different hybridization properties.
Still, as in the case of Affymetrix, the spot is likely to become an outlier in the probe-set used to detect a single transcript and will therefore be discarded from the estimation of the averaged probe intensity for the transcript.
A region was considered as amplified if its regional averaged probe intensity was significantly higher than zero, which was the global mean after standardization of the LogRatio intensities within samples.
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