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Vocal nuclei and adjacent non-vocal areas were outlined and the average pixel density was calculated using the Photoshop histogram function.
GRP-immunoreactive fiber pixel density was quantified as the average pixel density in three regions of each animal, the SPN, DGC and DH, and was calculated as the ratio to the density seen in the DH in control males.
The average pixel density of the two images was calculated.
The average pixel density (mean fluorescence intensity, MFI) within the selected area was calculated.
Developed films were scanned, and the average pixel density for each band was measured.
Fast-Fourier transform of the average pixel density over time was performed to determine heart rate.
Similar(47)
Developed films were scanned, and the average pixel optical density (OD) for each band was measured.
The average signal (pixel density) of duplicate spots representing each protein was evaluated by ImageJ (NIH).
Briefly, 8-bit images were converted into binary images to calculate pixel density, and the average number of pixels for each group was analyzed for statistical significance.
Net integrated pixel density for each spot (an average of duplicate spots after subtraction of average background density) was determined by densitometry and analysed using Quantity One software (ISBE, Sheffield, UK).
The higher the pixel density the sharper the screen.
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