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Unlike the average normalized intensity data used for an anatomical meta-expression database, log2 fold-change data for treatment versus control have been introduced.
An average normalized intensity was then calculated on the basis of both the HbpR bands visible in Western.
Figure 3 (panels A and B) shows a plot of the average normalized intensity ratio from the twelve pre-malignant and malignant subjects for actin and myosin, respectively.
Genes were considered expressed when the average normalized intensity was higher than the background level.
Each dot in Figure 3(c) represents the average normalized intensity of an individual label in the candidate cluster(s).
Figure 9(d) shows some labels with average normalized intensity lower than Ipeak + 0.2; these labels looked faint all over.
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The average normalized intensities were used for biological replicates (MOCK, ETOH and 1 nM conditions).
All average normalized intensities of HbpR mutants were then plotted in a Box-plot, to identify outliers below the 25% and above the 75% quantiles (Text S1).
with respect to the average normalized intensities for each peptide.
The labels with average normalized intensities lower than Ipeak + 0.2 were eliminated in Step 6. Figure 3 d) shows the histogram of the normalized voxel intensity of the ADC map.
Differential gene expression levels were calculated per time point by subtraction of the average normalized intensities of control samples from those of the mdx samples to correct for normal aging processes.
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