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The most extensive poliovirus mutant frequency data set estimated an average mutant frequency of 2 × 10−4 (Acevedo et al., 2014).
However, the strategy is limited by the need to design primers in which one has a Tm that is substantially higher than the other; moreover, the average mutant frequency is approximately 80%.
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Despite a small average increase in mutant frequency for smokers, an increased proportion of base substitutions at AT base pairs in smokers (p = 0.2) hinted at a smoking-associated shift in the mutation spectrum.
The ratio of colonies to cells placed on the growth medium is the mutant frequency.
The mutant frequency was determined using the cII and the lacI mutant analysis systems.
It follows from the definition that v<1 reflects a decrease in mutant frequency over the period of competition, that is, the mutant is less fit than the wild type, while v>1 reflects an increase in mutant frequency, that is, the mutant is fitter than the wild type.
Mognato, M. & Celotti, L. Modeled microgravity affects cell survival and HPRT mutant frequency, but not the expression of DNA repair genes in human lymphocytes irradiated with ionising radiation.
Timepoints for determination of lacI mutant frequency were 10, 30, 90, and 180 days of exposure.
In average, mutant RCA were ~1.5 larger than control ones (~60° vs. ~40°) (Table 1).
3 lines were chosen for whole genome sequencing based on phenotype: J318 (mutant frequency 8%%), J328 (mutant frequency 4.3%%), and J327 (mutant frequency 2%%).
The frequency of mutants accumulated after 27 generations in the simulation agreed closely with the measured mutant frequency.
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