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The average molecular area was obtained according to the π A isotherms.
These latter studies revealed that the adsorption of sweeteners caused an increase of the average molecular area occupied by the lipids, and resulted in increased structural order of membranes.
To quantify the effect of Ch on the other lipids, the average molecular area of an ideal mixture at 3 mN/m was calculated and compared to the observed molecular area.
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The calculated average molecular areas of SLPE/Ch monolayer at surface pressure of 3 mN/m are presented in Table 2.
The mean molecular area declined as the surface pressure increased.
Assuming an average DMPC molecular area of 0.6 nm2 [39], [39], and an apoLp-III helical thickness of 1 nm [37], the average number of DMPC molecules per NLP extrapolated from the NLP diameter as measured by AFM were 717±55, 424±75, 217±27, and 99±25 nm for NLP-1, NLP-2, NLP-3, and NLP-4, respectively (Table 1).
The model consisted of three descriptors: Cross-head Speed (CHS), Number Average Molecular Weight/Main Chain Surface Area ratio (Mn/SAMC), and Normalized Main Chain Mass (nMMC).
As a result, five descriptors were found to be important in predicting the kOH values which related to the electronegativity, topological polar surface area, double bonds, average molecular weight, and halogen atoms in the molecule.
The weighted average molecular weight was obtained by integrating the peak area of the c(M) distribution.
It is noted that the observed change in average tilt angle with increasing coverage produces a coverage-dependent molecular area that is not taken into account in the BET or any other known theory of multilayer adsorption.
At t = 0.5 min, enzymatic denaturation of the collagen structure was fairly moderate and the calculated molecular area (see Supporting File S1 and Supporting Figure S7 for details) exhibited an average value of 4.8±2.2×104 Å2.
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