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The average microvessel density of each tumor suggests that new vessels were scattered throughout the tumors.
CD31-immunostained sections were examined and regions of high and average microvessel density were selected.
Quantification of stained vessels can be achieved by measuring highest microvascular density (h-MVD), the average microvessel density (a-MVD) or the microvascular volume (MVV).
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Statistical analysis showed that the averaged microvessel density, expressed by the CD31-positive counting per 200X-view field for the MSeA-treatment and the control group, was 54±4 and 87±19, respectively.
Median microvessel density (MVD) was 47 (mean, 49; range, 15 97) microvessels per mm.
The median microvessel density (MVD) was 22 (range 7 69).
Median microvessel density irrespective of degree of inflammation was 71.
For the microvascular marker (CD34), DAB staining was quantified within whole tumor areas using the Color Deconvolution algorithm (Aperio) for standard area quantification as well as the Microvessel algorithm (Aperio) for quantification of alternative metrics that may be prognostic (average vessel area, average vessel perimeter, average lumen area, average vascular area, and microvessel density).
The mean visual microvessel density was calculated as the average of four counts (two of the authors: two microscopic fields).
A short average distance between vessels indicates high microvessel density.
All image pixels were sorted to determine their distance from the nearest vessels and the average distance between vessels, which reflects microvessel density, was reported.
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