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The cutoff labeling index was 25% for p27 (the average labeling index for malignancy), 30% for Galectin-3, and 10% for HBME-1 (the respective average labeling index for benign).
The average labeling index of Nrf2 was assessed according to the proportion of positive cells in each field.
The average labeling index for normal/benign and malignant thyroid lesions (Fig. 2, upper panel) suggests a threshold.
The average labeling index of caspase 3 and TUNEL was assessed according to the proportion of positive cells, after scanning the entire section of the specimen.
When Galectin-3 and HBME-1 signals are added together, a new threshold of 40% is suggested, which is the average labeling index for normal and benign (Fig. 2D).
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The average labelling index of BMP-7 was assessed according to the proportion of positive cells in each field.
The average labelling indices with MIB1, a proliferation marker, were 14% in CPC and 3.7% in CPP (Vajtai et al, 1996).
Data are presented as mean labeling index or as labeling index against cell position.
In PCa specimens average Mcm2 expression was greater than Ki67 and geminin expression (median labelling index (LI) 36.7, 18.1, and 3.4% respectively), consistent with their differential expression according to growth status (P<0.0001).
To generate the best fitted-regression lines we applied the Linear Regression Model of the Prism software (version 5; GraphPad software) to the averaged labelling indexes of the time points before reaching the GF.
The average Ki-67 labeling index (LI) was 5.3% in pre-RT samples and 11.54% in post-RT samples.
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