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The average intensity was evaluated within the red channel in each image after performing the background removal.
Upon application of UV irradiation (λ = 254 nm), in which the average intensity was 0.1 mW · cm−2 at the working plane, the effect of TiO2 Ws for K562 cell proliferation in the presence of UV irradiation for 180 s was investigated.
The average intensity was determined in the photobleached region, before photobleaching and post-photobleaching.
The average intensity was then determined for each cell, and normalized to control transfected cells (β-gal plus either empty FLAG vector or pSUPER).
Candidate tags were selected on the basis of statistical criteria (see Materials and methods) limiting our analysis to tags whose average intensity was higher than log210.
Average intensity was recorded along a line (scanwidth of 10 pixels) along the antero-posterior axis of the embryo, mid-way between the dorso-ventral axis.
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The corresponding time dependence of the average intensity is shown in Fig. 6(b).
In [14], the segmentation of masses by detection of concentric layers, using progressively lower average intensity, is proposed.
Xl-PSIDs with the lowest average intensities were grouped in the first decile while those with highest average intensity were grouped in the 10th decile.
Null values for A (average intensity) were excluded from the statistical analyses.
The size, shape, and average intensity are considered to identify and remove artifactual nuclei.
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