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A Nextera DNA library with average insert size approximately 450 bp was constructed for each species following manufacturer's instructions (Illumina).
This BAC library contained 182,784 clones with an average insert size of about 104 kb.
The draft phase involved two clone libraries, one small insert library (2200 bp average insert size) and one medium insert library (6289 bp average insert size).
A library of several thousand clones was generated, with an average insert size of 1.5 kb.
All libraries are at approximately 5× coverage with an average insert size of 39.5 kb.
The average insert size of the entire library is estimated at 96 kb.
The average insert size of 12 clones was 1.7 kb (based on PCR of inserts).
The final assembly contained 27,377 reads: 12,990 small insert reads (average insert size ≅ 4200 bp), 7,364 large insert reads (average insert size ≅31,650 bp) and 7,023 PCR-based reads.
The HindIII library (BD_ABa) consisted of 36,864 clones with an average insert size of 128 Kb (Table 1), and the EcoRI library (BD_CBa) consisted of 36,864 clones with an average insert size of 124 Kb (Table 1).
Their average insert size was 1.2 kb.
The average insert size for both libraries was 1.5 kbp.
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