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Subsequently, images were subject to densitometry analysis using the Fiji software by measuring the average grey values of the pixels of the first 20 somites.
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AOS schemes with large time steps still reveal average grey value invariance, stability based on extremum principle, Lyapunov functionals, and convergence to a constant steady-state [31].
Finally, the mean fluorescence was calculated as average grey value in selected regions in the nucleus and cytoplasm in individual cells after background correction.
Degree of pigmentation was measured as the average grey value (0 = black; 255 = white) of a 0.04-cm area of each of both dorsal hind wings, with wing scans converted to 8-bit images.
The degree of basal melanization of each dorsal forewing was quantified as the average grey value (scaled from 0, i.e. black, to 255, i.e. white) of the area of the discal wing cell (cf. Talloen et al. 2004).
The average of mean grey value was determined in arbitrary units in each experimental condition, and the results were expressed as a percentage of control.
(P ) Western-blot quantification (mean grey values).
Depicted values represent quantification of mean grey values of corresponding bands.
400× images were taken along the length of the neurite side of the platform using a confocal microscope, inverted to grey scale images of the same threshold, and optical density was determined as mean grey value in pixels using Image J software (U. S. National Institutes of Health, Bethesda, Maryland, USA) and averaged across groups.
The mean grey value in CSF was higher in both types of hydrocephalus; obstructive (14.7% mean grey value) and communicating hydrocephalus (38.3% mean grey value) when compared to the control CSF (6.9% mean grey value).
We analysed the mean grey value of each image.
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