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Each numeral in the image corresponds to the average gray intensity value of each shape.
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The mean gray intensity of the entire image was also calculated in ImageJ and used to subtract an average background value from the invaded spot.
The contrast is considered to be optimal if the foreground mean gray intensity is 50 to 80% of the background mean gray intensity.
The contrast analysis segments the hematoxylin-stained cells (foreground) and analyzes their mean gray intensity, which is then divided by the background mean gray intensity.
For accurate nuclei segmentation, the image contrast must be relatively high; the foreground mean gray intensity should be 50 to 80% of the background mean gray intensity.
However, greatly underexposed images (blankfield image mean gray intensity <200) as well as overly overexposed images (blankfield image mean gray intensity >250) may cause false labeling indexes.
Further, Figure 11 shows the effect of air pressure on the average gray scale intensity of the image.
Fluorescence intensity (signal intensity) was calculated as the average gray value within the selection.
The mean value of the gray intensity for each extracted pixels group is computed.
Telomere fluorescence intensity was measured as "average gray value" (total gray value per nucleus area).
Cy3 fluorescence intensity (telomere fluorescence) was measured as "average gray values" (total gray value/nuclei area) units (arbitrary units of fluorescence).
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