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The average fragment lengths that were estimated from data are also given in the table.
About 5 μg of each BAC DNA were individually fragmented for 1 min at 3 bar N2 by nebulizers (part of GS DNA Library Preparation Kit, Roche Diagnostics) resulting in average fragment lengths of 700-800 bp.
RNA-seq (Wang et al. 2009) paired-end libraries with average fragment lengths of 250 bp were constructed, and each library was sequenced on a single lane of an Illumina GAIIX sequencer flow cell (Illumina, San Diego, CA) to generate a minimum of 3 gigabases of 75 bp, paired-end sequences.
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Assuming that the residual stresses were negligible, in the initial stage of fragmentation, the average fragment length was related to the stress acting in the coating.
After DNA fragmentation and isolation (average fragment length 189 bp), 6,345,662 reads (75 bases per read) were generated using the GAIIx sequencing system (Illumina, San Diego, CA, USA).
To obtain fragmented cross-linked chromatin with an average fragment length of 750 bp, a sonication step was performed consisting of about 30 pulses of 10 s each (with 10 s rest between each) at 50% power in an ice bath (Labsonic M sonifier, Braun Biotech international).
The average fragment length is ℓ 0-α, where a normalizing factor (ℓ 0) of 1 μm was chosen.
The derived theoretical average fragment length dependence on applied load is used to determine the fibre strength distribution parameters and the effective interfacial shear stress for carbon/epoxy single fibre composites with different fibre surface treatment and for glass/vinylester single fibre composite.
When the average fragment length can be accurately inferred (either computationally or empirically), the combined density will form a single peak where the summit corresponds closely to the binding site.
NEXT-peak can estimate the average fragment length, even if the experiment does not measure the average fragment length.
In Figure 1, the haplotype length n = 100 and the average fragment length l = 3.
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