The total carotenoid content of the samples was calculated according to the Lambert-Beer's law (average molar extinction coefficient: 100,000; average molar mass of carotenoids: 600).
For this reason, lipid quantification was assessed by using an average of extinction coefficients for all fatty acids (Figure 3): y = 1.327 × 10- 2 x + 2.911 × 10- 2 (R2 = 0.999), where y is the absorbance measured at 440 nm and x is the fatty acid concentration in milligrams per liter.
The average nonreduced cysteine extinction coefficient at 280 nm was 60.51, the nonreduced cysteine absorption was 0.91, the reduced cysteine extinction coefficient was 39.07, and the reduced cysteine absorption was 0.90.
The experimental results demonstrate that the average refractive index and extinction coefficient calculated by two-step film envelope method are more accurate than those calculated by the traditional envelope method.
Pepstats calculated molecular weight, isoelectric point, charge, size of protein, extinction coefficient and average residue weight for all the proteins.
Physicochemical properties of the protein were calculated by the ProtParam server including AI, instability index (II), pI, extinction coefficient and average hydropathicity.
The properties including aliphatic index (AI), GRAVY (grand average of hydropathy), extinction coefficients, isoelectric point (pI), and molecular weight were analyzed using this tool.
where C is a constant, ε is the molar extinction coefficient, E g is the average band gap of the material and n depends on the type of transition.
