Sentence examples for average expression ratio from inspiring English sources
To correct for inter-experimental error, all 790 probes were standardised between the two experiments by dividing the 790 genes from the original dataset and the new dataset by the average expression ratio per probe in Microsoft excel (Microsoft Corporation, Redmond WA, USA).
To correct for inter-experimental error between original and control datasets, all 790 probes obtained in the stringent analysis were standardised between the two experiments by dividing expression values of the 790 genes from the original dataset and the new dataset by the average expression ratio of the two datasets per probe in Microsoft excel (Microsoft Corporation, Redmond WA, USA).
The average expression ratio of Cy5 to Cy3 was obtained for each gene.
For each replicated dye-swap, the average expression ratio of a given gene is calculated as the geometric mean of the two ratios [ 62].
Furthermore, the average expression ratio of miRNA targets (0.997) was significantly lower than that of the non-miRNA-target genes (1.021, P < 10-100, testst, dataset 1).
Conversely, RASSF1C expression level in PETs was significantly higher than that found in normals in 11 of 13 cases (P = 0.001), having a fold average expression ratio PET/normal of 11.4.
The differentially expressed genes were selected and the average expression ratios were calculated from the log2ratios of the two dye-swap experiments for further use.
Microarray data were validated by qRT-PCR on 11 differentially expressed genes with different functions and by comparing their average expression ratios (DS/controls) with those of the array (Additional file 3).
The experiment was done in duplicate and the average expression ratios of the two runs are shown.
We next calculated the average expression ratios (test/reference) in all analyses.
Average expression ratios for a control plasmid containing the U6 promoter was set to 100%, and relative expression levels for other samples calculated accordingly.
