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Furthermore, the therapeutic utility of their findings was limited by the inability to predict activation status of relevant oncogenic pathways in a given individual tumor specimen, as opposed to presenting average expression patterns in predefined tumor subgroups.
In silico coexpression analysis from PrImR average expression patterns was performed using built-in functions in Fiji and Imaris.
(A – D and F – I ) Average expression patterns of neurotransmitter marker genes and r-opsin1 projected onto a common whole-body nuclear reference template.
For PrImR, average expression patterns at 48 hpf were obtained after image registration of in situ confocal scans on a common reference axonal scaffold, as described in [ 47].
The reference mosaics represent the average expression patterns for all patients in each class and demonstrate distinct expression patterns for the patients with sepsis compared with the patients with SIRS.
Fig 4A shows the average expression patterns for genes regulated by both GFs and reveals that a considerable number were less potently induced (or not induced) by HRG in the HB4a cells (Fig. 4A; clusters 2 & 3).
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The average expression pattern of all the genes in these clusters is depicted in Figure 3A.
In the PCA approach, the first basis vector captures the average expression pattern of the member genes, and the first principal component can estimate the presence and the strength of this pattern in a gene expression profile.
We registered minimum four individual larval whole-body scans for each gene to create an average expression pattern.
The average expression pattern of the former group is down-regulation and the latter, up-regulation during development.
Additionally, a gene expression profile for each module is available visualising the average expression pattern (module eigengene) for each sample of analysed experiments.
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Justyna Jupowicz-Kozak
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