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From the bottom sample data, an average dilution factor of 5003.00 was calculated (Fig. 11).
The average dilution factor was 10 – 20.
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The number of cells was determined by averaging the number of cells in four squares and multiplying this average by a dilution factor.
Total cells = (average count/square) × (dilution factor) × 104 × (predilution volume).
In a second step, all signal intensity ratios were normalized to the ratios obtained from the equimolar subpool-0 of each array and averaged for every dilution factor.
The cells/ml was calculated as average cell count x dilution factor x 10 cells/ml.
The observed dilution factor averaged across the three rounds of recovery was therefore 4 ± 1, similar to the result calculated for N. tabacum in the indigo carmine and peroxidase studies, and suggested another 4% of the expressed E1cd activity may have been recoverable from additional rounds of recovery.
Plaques were counted, averaged and multiplied by the dilution factor to determine viral titer as Pfu/ml.
The raw numbers were multiplied by the appropriate dilution factor and averages calculated for each point.
The C Ts of each triplicate were averaged and plotted against the dilution factor.
The log10 average population and the cfu/mL of the average of the duplicate counts was calculated as log10 (Ci × 10-D) or (Ci × 10-D), respectively, where Ci = average of two plate counts and D = dilution factor of the plates counted.
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