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The average current amplitude of compound EPSCs necessary to trigger a spike at threshold level was maximal (mean = 3.3, s.e.m. = 0.04 nA; n = 10) for EPSCs with fast kinetics (τrise/τdecay 0.1/1 ms), and gradually decreased when the EPSCs became slower (Figure 3E).
(B ) The average current amplitude of IOCA2 normalized to control conditions (<1% DMSO) was similar when DIDS, NFA, or NPPB was bath applied at 100 μM or 300 μM (n = 3 4 endolysosomes for each).
The results showed no significant difference among the various groups of oocytes tested for a particular agonist, although average current amplitude was consistently smaller for LPAR stimulation, and larger for M1-stimulated responses.
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(B ) Average current amplitudes plotted over the indicated potentials (n = 4, Error = ±SEM).
(B ) Average current amplitudes normalized to maximum current for cells expressing ANO6 (red square) or ANO1 (black circles) plotted vs time after establishing whole-cell recording.
(C ) Average current amplitudes ± SEM at +100 mV for 1-6-1_D554-K588 (red circles) and 6-1-6_N525-I527 (black squares) plotted vs time after establishing whole-cell recording.
The sEPSC amplitudes were estimated by subtracting the mean current amplitude recorded in the 5 ms before the event from the average of five data points at the peak of the event.
All the I-V curves presented in Fig 2 were the averaged current amplitudes from a group of cells (n = 4 6).
The persistent current of Nav1.8 was evoked by depolarization for 200 ms, and measured by averaging the current amplitude recording of the last 10 ms for each pulse (Fig. 2A).
As above, we made voltage-clamp recordings in MSNs and delivered light pulses through the microscope objective (Fig. 2E), evoking EPSCs with an average (n = 5) latency to 4.1±0.3 ms. From a holding potential of -70 mV, the average peak inward current amplitude was 50.5±17.3 pA, while from a holding potential of +40 mV, the average current at 150 ms was 28.2±6.4 pA (Fig. 2E,F).
The average minimal depolarizing current amplitude required to elicit spike discharge (rheobase) was significantly lower for MSN in dopamine-depleted animals (62.5±6.2 pA, n = 8) than the current to threshold for neurons in untreated control animals (91.25±2.95 pA, n = 8, p<0.05; Fig. 2C).
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