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Assuming an average cellular volume of 1 pl, the concentration of free NEDD8 in U2OS cells was ~29 μM and that of free ubiquitin was ~25.6 μM.
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The mean cellular volume also changes with X.
In this study, we defined the signal average, cellular density, average dynamic range, and cell volume as characteristic parameters of single cells.
The last column of Table 1 shows the calculated number of autophagic structures in a real cellular volume of 5000 μm, an average rat liver hepatocyte [ 4].
At this time point, the absolute amount of accumulated MPP+ was measured and the cellular volume of IMAs was determined (2.5 pl/cell) so that average intracellular MPP+ concentrations could be calculated (Fig. 1F).
This method illuminates approximately 200 nm from the glass surface attached to the bottom of the cells; but, in the same section (and elsewhere), it is stated that an average of 5 RTC/PIC (and up to 30) objects were detected using spinning disk confocal (to image the complete cellular volume).
We found that the intrinsic cellular fluorescence, Fcell, is correlated to cellular volume, Vcell (Figure 10).
In the simulations the cellular volume is discretized and represented by a three-dimensional cubic lattice.
Physiologically, crowding agents and cosolutes occupy 20 40% of cellular volume.
Further, older cells have large vacuoles that occupy most of the cellular space and their cytoplasmic volume is considerably smaller than the total cellular volume [84].
Inspection of the plots shows no compelling evidence for differing bias with increasing average volume or average volume change.
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