The phrase "average background fluorescence" is correct and usable in written English.
It can be used in scientific or technical contexts, particularly in fields like biology or chemistry, to refer to the typical level of fluorescence emitted by a sample or environment when no specific fluorescent markers are present.
Example: "The experiment measured the average background fluorescence to ensure that the results were not skewed by ambient light."
Alternatives: "typical background fluorescence" or "mean background fluorescence".
The average background fluorescence was subtracted and the ratio of fluorescence in a degradation-system-expressing cell to a neighboring control cell was calculated.
Readings from regions without nuclear staining provided average background fluorescence for each tumour section.
The fluorescence intensity inside each nucleus and the average background fluorescence intensity were determined for both the YFP/GFP and TRITC channels.
The average background fluorescence of wild-type (GFP-negative) embryos was subtracted, then values were normalized to the average value of the GFP-SAS-5 wild type intensity.
Local average background fluorescence was subtracted and the signal for each gene was normalized to a housekeeping gene (gpdh) that showed consistent expression between genotypes on the arrays.
Cytotopic CD59 at a final concentration of 0.7 μM was incubated with 200 μl of fluorescently-labeled liposomes in the presence or absence of peptide, and the average background fluorescence intensity was measured for 10 min.
PBS buffer, spotted on either side of each protein spot was used to detect protein carry-over during spotting and fewer than 10% of PBS spots showed signal intensities higher than the average nitrocellulose background fluorescence intensity (FI) value (see Materials and Methods).
The plots of the allele concentrations in spotting solution versus average background-corrected fluorescence intensities for the six SNPs were analyzed using the Langmuir-type model (Eqs. 12 and 13).
Correcting for background fluorescence, average fluorescent intensity was measured for each labeled protein.
The intensity of each spectrum was then averaged over approximately 400 s, following subtraction of the averaged (100 s) background fluorescence.
The collected spectra were averaged and the background fluorescence was subtracted using an asymmetric least squares smoothing.
