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For each individual sample group or treatment condition, the background-subtracted average absorbance value was normalized to that of the untreated ("NT") control group to obtain the normalized cellular viabilities.
A sample was considered positive if the average absorbance value of the three replicate wells was four times greater than the average absorbance value of healthy uninoculated samples of non-transgenic plants analyzed in the same plate [ 39].
The average absorbance value was detected and then applied to the mean standard curve like the one shown in Figure 2 to obtain a serum cadmium concentration.
Cell viability was calculated as percentages of the positive control: Cell viability = Average absorbance value of each triplicate positive control × 100.
Synthesis of TNF-α was statistically elevated at 1 × 10 μg/mL, although the average absorbance value (8.8 pg/mL) was below the minimum limit of detection for the assay (10 pg/mL).
Stimulation index of the lymphocyte proliferation (SI) of experimental mice was calculated according the following equation: (4) S I = X ¯ A b s T w - X ¯ A b s C w C u t o f f where X ¯ Abs Tw and X ¯ Abs Cw stand for the average absorbance value for the antigen treated wells and the average of the average absorbance value for the control wells (only DMEM), respectively.
Similar(54)
The results were expressed as the average absorbance values of four replicates.
These correlations coefficients correlate the average absorbance values of all 12 scans of an admixture to the individual absorbance values of each scan of that admixture.
Calibration curves were prepared everyday for 3 days, and the average absorbance values were used in constructing the working calibration curve.
where, r = correlation factor, X = absorbance values of scan A of admixture/paint, ( overline{x} ) = average of the absorbance values of scan A of admixture/paint, Y = average absorbance values of all scans from all three batches of admixture/paint ( {bar{text{Y}}} ) = average of the average absorbance of all scans from all three batches of admixture/paint.
Average absorbance values were calculated.
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