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Since HRV-QPM protein and protomer structures were inferred by homology to their closest available relatives, these capsids (HRV-16 and HRV-14) were produced from the available empirical data, using the same approach.
One hundred and eighty-six cases were considered not eligible because they had no available relatives.
Affected individuals and their available relatives were recruited with informed consent according to an IRB-approved protocol.
We also genotyped available relatives of the mutation carriers to determine whether these mutations segregate with the diagnosis of AD.
Variants that were selected for further analysis were validated using Sanger sequencing and analyzed in all available relatives.
Of the remaining 36 families, the pathogenicity was supported in 21 by a full segregation in available relatives.
For each proband the clinical and molecular examinations were extended to the available relatives at various degrees of kinship.
The estimated breeding value (EBV) expresses the genetic merit of a breeding animal estimated based on their own performance and performances of all available relatives.
Data were collected on 236 dogs from one large family, starting with a deaf proband and her immediate relatives, then extending broadly to available relatives.
Using information on all available relatives may improve individual mutation predictions as opposed to restricting information to second-degree relatives only.
BRCAPRO does not consider FH information on relatives more distant than second degree, whereas the present model can incorporate all available relatives.
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