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Using all the available full length sequences, we found 13 conserved sites that had not been previously reported (Table 1).
Comparison to available full length cDNA sequences and Open Reading Frame (ORF) analysis of camel sequences that exhibit homology to known genes show more than 80% of the contigs with an ORF>300 bp and ∼40% hits extending to the start codons of full length cDNAs suggesting successful characterization of camel genes.
In order to determine whether the di-arginine motif regulates a diverse set of proteins in the cell, we randomly selected two further membrane proteins identified in the bioinformatic screen as containing a di-arginine motif in the first 25 residues, and which had an available full length cDNA, and carried out site-directed mutagenesis of the di-arginine motif to glutamic acid.
Whenever circulating DNA is analyzed, copy numbers derived from a real-time PCR assay with fragmented DNA as a template cannot easily be interpreted as haploid whole genome equivalents because the real-time PCR assay counts only the copies of available full length target.
Site-models 0, 1, 2, 3, 5, 7, 8 from codeml were tested on the available full length P450 and UGT sequences with likelihood ratio tests [ 38].
The distances between the empirical points correspond to distances between the user amplicon sequences, using the best available (full length 16S) sequence data to correct for distortions caused by the choice of individual markers.
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We have performed the first phylogenetic analysis to include all available full-length HCV genomic sequences (n = 345).
The human TGF-β1 5'UTR was compared to the available full-length sequences from chimpanzee, pig, rat, and mouse (Figure 4).
Two studies comprehensively analyzed all available full-length HBV genomes in GenBank/EMBL/DDBJ and reported the frequent occurrence of recombination event between two or more HBV strains [14], [15].
We expect that in DNA of less than 300 bp, the number of available full-length 187 bp target sequences is reduced to less than 38%% when assuming that the DNA fragments were generated by random cuts (see Supporting Information File S3).
A multiple sequence alignment of p22 and its homologues from the 72 available full-length human norovirus sequences from viruses classified in different genogroups (GI.1 to GII.12) found 13% amino acid identity between all proteins (summarized in Figure 5A).
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CEO of Professional Science Editing for Scientists @ prosciediting.com