Sentence examples for available from Invitrogen from inspiring English sources

Custom formulations are also available from Invitrogen.

pcDNA3.1 is available from Invitrogen (cat. #V79020).

Hes1and HesiRNARNA sequences were 5'- CGGCCAATTTGCCTTTCTC-3', 5'-GCCCTGGGATTACAAGGAT-3', respectively, predicted using the online software BLOCK-iT™ RNAi Designer available from Invitrogen (https://rnaidesigner.invitrogen.com).

Qdots are currently available from Invitrogen in their unconjugated form; Invitrogen provides a detailed protocol for conjugation of Qdots to phalloidin on their web site.

Plasmid DNA was sequenced using Big Dye chemistry (Applied Biosystems) with the M13 Forward and M13 Reverse primers available from Invitrogen and/or with an additional forward primer DONRF-2 (Table 2).

siRNA sequences are available from Invitrogen (Stealth Select).

siRNAs were available commercially from Invitrogen (Esrrg siRNA, 10620312) and Ambion (Ush2a control siRNA, AM16704).

Levels of selected cytokines were determined using commercially available ELISA sets purchased from Invitrogen (TNF-α, TGF-β, IL-10; Invitrogen Corp).

Alamar blue assay is used in high throughput screens for antimicrobial agents [85] and is available commercially in kit form from Invitrogen.

In-vitro mutagenesis by PCR-mediated recombination44 was performed as detailed before19, 20, 45 using the cDNA of ggTas2r1 cloned in the vector pcDNA5/FRT (Invitrogen) available from previous experiments9 as template.

Five clones of each were selected and sequenced on the ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) using the vector primers available from the cloning kit (Invitrogen) as sequencing primers, to ensure correct insertion of the amplicons into the plasmids.