The selected papers were searched and, where available, data were extracted with respect to: Research methodology employed.
Soil available potassium (K) was extracted with ammonium acetate and measured using flame photometry (HG-5, Yinze instrument equipment co. LTD, Shanghai, China)48.
We have also shown that DNA extracted with commercially available kits, although not optimal because of non-specific DNA damage due to the extraction process, is suitable for a sensitive enrichment of DNA damage since 5×107 haploid copies of the yeast genome is sufficient.
High-molecular-weight DNA was extracted with commercially available PureGene Kits (Gentra Systems, Minneapolis, MN) from the white blood cells of archived maternal and umbilical cord blood samples.
Available P forms (Olsen P) were extracted with 0.5 mol L−1 NandO3 and measured by spectroscopy.
DNA was then extracted with a commercially available kit (DNeasy Blood & Tissue Kit, Qiagen N.V., Venlo, The Netherlands).
Cells were stored at -80°C until total RNA was extracted with a commercially available kit (NucleoSpin® RNA II mini, Macherey-Nagel, Germany, Cat. 740955) following manufacturer's instructions.
All OGs that contained at least one sequence of a representative of each group were extracted with Perl scripts (available upon request) and concatenated into seven supermatrices that constitute the seven decisive datasets.
Total RNA was extracted with two commercially available kits depending on the amount of cells in the starting material: First, the Total RNA Isolation Mini Kit (Agilent Technologies, Waldbronn, D) was used for RNA extraction from cultivated tumor cells, from homogenized tumor tissue and from PBMC enriched by Ficoll-Paque™Plus density gradient centrifugation.
The available heavy metals in soil sample were extracted with AB-DTPA reagent and determined through AAS.
The datasets analyzed during the current study are available in the Qiita repository with the specific study accessions in Supplementary Data 1, and were extracted with Qiita's redbiom interface.
