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Nor were there readily available expressions for the equivalent vector potential distribution.
The significance of the correction factor and the accuracy of the different available expressions is illustrated for several cases of practical interest.
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These systems offer a practical, effective alternative to currently available expression systems in the Drosophila research community.
Although intein methods can greatly simplify protein purification, commercially available expression vectors still rely on conventional restriction/ligation cloning methods for target gene insertion.
The vector, which is a modified version of the commercially available expression vector, pET-32a, contains an N-terminal thioredoxin fusion protein and a hexahistidine tag, and is adapted to the Gateway expression system.
We use the currently available expression datasets (in situ hybridization images & qPCR time series) for a basal anthozoan the sea anemone N. vectensis to construct continuous spatiotemporal gene expression patterns during its early development.
Therefore, we surveyed the available expression data for these genes.
We analyzed publicly available expression data on normal human tissues.
On the basis of available expression data we predicted that hBD-2 and hBD-3 would be the best candidates.
We have validated our approach by interpreting several publicly available expression microarray experiments using the Atlas of Signaling pathway.
Where multiple probes were available, expression was averaged.
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