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For gene expression assays, forward primer sequences for miRNAs were taken directly from the sequence information provided by Salem and colleagues [ 13], or, if not available, designed based on miRNA sequences found in the trout genome.
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Many available TKR systems are designed based on the four-bar link theory and feature J-curved femoral components.
Firstly, a model-based nonlinear observer in the proposed method is designed based on available information.
Cichlid-specific PCR primers were designed based on available and/or draft genomic and transcriptomic cichlid sequences, which were identified by tblastx searches of publicly available dlx sequences from other teleost species (see Additional file 1 for species and accession numbers).
Management scenarios are designed based on available technical data and stakeholders' perceptions.
The chimeras were designed based on available structural data to maintain high-affinity binding to type II receptors.
The recombinant NDV-H9Con virus expresses a consensus-H9 hemagglutinin (HA) that is designed based on available H9N2 sequences from Chinese and Middle Eastern isolates.
The primers were designed based on available sequences (Table 1) using Primer3 software [ 36].
Methods and Results: Internal primers were designed based on available sequences of the plastid ndhF gene and the rpl32-trnL intergenic spacer region, and the nuclear gene PepC.
Primers amplifying 7b (736 bp), membrane protein (575 bp), polymerase (386 bp), and spike-NSP3 (1,017 bp) were designed based on available feline coronavirus sequence (1, 12, 13 ).
Specific primers for each gene were designed based on available genomic or cDNA sequences and on the alignment with related genes to avoid cross-amplification, using Beacon Design software (Premier Biosoft Int., Palo Alto, CA).
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