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These results are easy to use and complement those given by previously available deficiency theorems.
To conduct quantitative complementation tests on chromosomal deletions, a set of available deficiency lines were ordered at the Bloomington stock center (http://flystocks.bio.indiana.edu).edu
Using the available deficiency strains at the time with deletions from 46B to 52D to do the complementation tests, no deletion was found to remove the Inr-a mutation.
Previously, characterization of genetic deficiencies has been performed by fairly low resolution or labor intensive techniques such as genetic linkage mapping, PCR analysis [ 7] and, more recently, by the application of snip-SNP [ 9, 10] and as a consequence many available deficiency strains remain poorly characterized.
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We did not work with all available deficiencies because some stocks gave variable genetic results or were not sufficiently healthy for our phenotypic analyses.
To map the locus or loci responsible for the phenotypes described above we began by analysing all of the available deficiencies, which uncover genomic stretches mapping to this region to which we will refer as the "classical deficiencies" (Figure 2 and see Table S1 for a full list).
We screened approximately 200 available deficiencies and identified 11 genomic regions that when deleted dominantly lead to sterility or semi-sterility of endos heterozygous females.
Marf alleles complemented with all the available deficiencies covered by Dp(1 Y dx5,y/C(1)M5 except Df(1 Exel6239 (Parks et al., 2004; Cook et al., 2012).
Among the mutations we failed to map, one maps to a region of the third chromosome without available deficiencies and was not pursued.
The available phosphorus deficiency in soils of our study area may be due to the inherent low-P status of the parent material and erosion loss.
The final result of the grant was a scientific publication (Seelen et al., 2005, J. Immunol. Methods 296, 187 198) and a commercially available complement deficiency screening kit, WIESLAB® Complement system Screen.
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