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Targeted sequences are mapped to the host genome (generally, the single reference genome available) based on sequence similarity.
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Of the 178 HIV-1 positive infants as determined by DNA PCR on FP-DBS using all the 3 methods (Roche Amplicor® and 'in-house' gag and pol assay), 110 infants had HIV-1 subtype data available based on pol sequence.
However, this strategy can also be applied to any non-traditional model organism (or group of species) for which there is an array platform available, based on DNA sequence similarity between array features and annotations (which are primarily derived from standard model organisms).
This information is provided from the literature when available, or based on sequence similarity.
The genomes of most fish species of economic importance are now available and annotation based on sequence homology with characterised genomes is underway.
Usually, the functionality of new identified cell wall genes in grasses can only be inferred from the information available from Arabidopsis or rice based on sequence homology searches.
$Primers were designed based on sequence available in GenBank.
The MR1-1 scFv was artificially synthesized based on sequence available from Genbank (accession no. U76382).
Algorithms are now available to group evolutionarily related proteins based on sequence similarities (see [ 11, 12] for recent reviews).
Gene-specific primers for rat Nav channel isoforms, α-actin and GAPDH were designed based on sequences available through PubMed (for sequences see Table 1).
PCR primers were based on sequences available at GenBank (AB076001– and23991) and were designed to amplify the non-coding regions of TTSuV1 and TTSuV2.
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