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Abundant availability, unique mucoadhesivity, inherent pharmacological properties, and other beneficial biological properties such as biocompatibility, biodegradability, low toxicity, and low immunogenicity make chitosan an exceptionally attractive NM for targeting therapeutics [ 48, 49].
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We subsequently explored the feasibility of altering the prototrophic marker sequences of other commonly used pRS plasmids to both increase the availability of unique sites in their polylinkers as well as to standardize unique site selection across the series.
Administrative databases include patient specific data but may, in some cases, limit the availability of unique patient identifiers.
The hospitalisations dataset did not contain names and addresses, and there was limited availability of unique identifiers in either dataset.
Other limitations include multi-step ligations and the availability of unique restriction sites in the insert and vector.
Traditional DNA cloning suffers from several limitations, including poor ligation efficiency, along with a dependence on the availability of unique restriction sites in both the insert and vector.
Overall, we argue that anthropogenic changes in nutrient availability present unique and powerful opportunities to test fundamental questions about life-history evolution (Table 1: H1–6).
We sought to develop and validate a method for linking a cohort study to a state-wide hospital admissions dataset with limited availability of unique identifying variables.
This pilot study has significantly increased the potential of a cohort study to determine health outcomes related to hospitalisation, even with limited availability of unique identifiers.
Firstly, they depend on the availability of unique and compatible restriction sites in the vector and the DNA fragment(s) to be cloned.
Tracking back 3 years incurred less misclassification than the 1 year look-back adopted by Raine et al (2010), although any method depends on the availability of unique patient identifiers (no method is perfect).
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CEO of Professional Science Editing for Scientists @ prosciediting.com