At the micro-level, system quality factors included the availability of templates [ 25, 42], interface design [ 31, 36, 41, 44, 48, 50, 55, 57, 59] and technical performance (e.g. speed and reliability) [ 44, 55, 58].
Since the availability of possible templates for comparative modeling, model was created for only SMARCAL1.
In higher eukaryotes, preference for HR or NHEJ is often regulated by cell-cycle phase, most likely because of availability of repair templates.
In fact, since HR relies on the availability of undamaged templates to restore any lost sequence information, it can operate only if a sister chromatid in close contact to the damaged one is ready to be used as a template.
The discovery that flexible templates are effective in this system, and the availability of a template with a prime number of binding sites, open up many possibilities for the template-directed synthesis of larger macrocycles.
By and large, the quality of comparative modeling depends on two factors: the availability of reliable template structures and the extent of structural similarity between target and template, which in turn is determined by the extent of the similarity between their sequences (Chothia and Lesk, 1986).
Unfortunately, due to the easy availability of cheap template websites as well as stretched budgets and short timescales, research and planning falls by the wayside.
The advent of computerised imaging systems and the availability of digital templating software have simplified the pre-operative planning process but still require an appreciation of the limitations imposed by simple mathematical principles.
Homology modelling methods on the other hand, while relying on the availability of a template, can be run on a single desktop computer.
However, the two template-based methods, ThreaDom and FIEFDom, have an obvious difference between 'Easy' and 'Medium/Hard' proteins because of the different availability of the template hits in the two category of proteins.
In experiment 3, the combination of the number of ligase enzyme units and the volume of DNA template in the ligation mix was evaluated, since a possible higher yield of ligated probe pairs due to a higher concentration of enzyme might be restricted by the availability of DNA template.
