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The justice secretary said he had only ever been involved in one discussion on the availability of reading material behind bars – and that was to allow EL James' erotic thriller Fifty Shades Of Grey to be circulated in women's prisons.
However, democratization of a script appears to have more to do with the availability of reading materials and of instruction in reading and the perceived relevance of literacy skills to the readers.
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While 25 bp reads minimize MPE regardless of the availability of paired reads, the read length that maximizes r2 is 25 for paired reads and 50 for single reads.
For this study, for MZ twin pairs, we selected one child per pair based on the maximum availability of reading- and language-related trait data, or otherwise randomly.
This technology-bound issue can be resolved by the use of paired end reads in some cases but may require the availability of longer reads for the resolution of other cases.
Ultimately, advances in read length (up to 500 bp or more), and the availability of paired reads, could make possible true whole-genome shotgun sequencing of soybean and other crop plants at greatly reduced cost.
This approach strongly benefits from the availability of longer reads (such as from 454 data) or the sheer number of data reads provided by next-generation sequencing platforms (such as the Illumina HiSeq, etc).
The availability of individual reads from the strains of interest allowed us to compare the B and O genomes in both the directions (e.g., reads from B mapped to the consensus sequence of O and vice versa), which enabled us to filter the results and identify hcSNPs that meet the quality criteria for both mapping directions.
With the availability of increasing read lengths, the loss of sequence information will not have major impact.
Advances in sequencing technology and availability of longer read lengths might further reduce the need for enzymatic primer digestion.
The five filters are independent and are applicable based on availability of PEM reads, matched normal sample and genome build annotation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com