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Therefore, in the current study we employed F-Aβ40 to take advantage of the current advances in optical microscopy and the availability of probes to track the protein accumulation in various cellular organelles.
We investigated either gene and/or protein expression, according to the availability of probes, as summarized in Table 1.
The latter approach depends on identification of targets that are upregulated at angiogenic blood vessels as compared to quiescent normal blood vessels and on the availability of probes that bind selectively and with high affinity to such targets.
On the other hand Whole Genome Sequencing protocols ensure the availability of probes mapping on the entire mitochondrial genome, therefore some missing variants derived from the exomes analyzed are linked to an incomplete covering of any mitochondrial genomes.
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In the range of higher target concentrations (14 pM-1.4 nM), saturation occurs due to the finite availability of probe molecules.
However, the application of live cell imaging is limited by the availability of fluorescent probes compared with other techniques that utilize antibodies as probes.
The details of cardiac Ca2+ signaling within the dyadic junction remain unclear because of limitations in rapid spatial imaging techniques, and availability of Ca2+ probes localized to dyadic junctions.
However, the two datasets were not completely concordant and had several non-overlapping CNV loci, probably because the arrayCGH experiment has higher resolution due to the availability of more probes (400,000 vs. 54,000) but was performed in fewer breeds.
DGE sequencing is a powerful and reliable tool for accurately quantifying the absolute expression levels of both mRNAs and miRNAs and overcomes the drawbacks of detecting a limited number of relatively high-abundance transcripts determined by the availability of array probes [20].
Low cost, portability, ease of use, and wide availability of imaging probes are key advantages of optical imaging.
A limiting factor in the development of new molecular imaging techniques is the availability of suitable probes.
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