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It seems intuitive to assume that there are fluctuations, e.g. in the availability of nucleotides in the nucleus.
Deprivation of glutamine reduces also availability of nucleotides that leads to a small decrease in RNA accumulation compared to growth in high glutamine (at least until the 72 hours, Fig. 5B) and a more dramatic effect on DNA synthesis (Figure 1F, S1D).
In intracellular symbionts, relaxation of natural selection, lower recombination frequency, small effective population size, codon usage, availability of nucleotides in the cytoplasmic pool and loss of DNA repair genes may contribute to lower G+C content [ 4, 31, 32].
In addition to altered thymidine synthesis, purine biosynthesis is negatively affected by low folate (10-formyltetrahydrofolate), thereby limiting the availability of nucleotides for DNA synthesis and repair, which will also have consequences for genomic stability.
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The kinetics of hybridization will depend on the availability of the nucleotides on the probe and thus on the length of the oligonucleotide.
In the present study, we selected 33 inbred laboratory mouse strains, including 31 classical inbred strains and 2 wild-derived strains, based on the Mouse Phenome Database priority list and the availability of single nucleotide polymorphism (SNP) data.
Availability of single nucleotide polymorphism (SNP -based molecular markerSNP -basedtly benefit breeding efforts in such non-molecularcies.
Advances in genomic technologies and the availability of single nucleotide polymorphism (SNP) markers have enabled genome-wide studies of the effect of selection in cattle [ 1, 2].
With the increasing availability of single nucleotide polymorphism data, the scope has nowadays been extended to the search for events ascribed to positive selection [ 21, 25, 28].
The subsequent specialization of guanylate kinase may have facilitated greater availability of guanine nucleotide to replace inosine, thus avoiding ambiguous rules in DNA replication that would have been achieved with this consolidation stage of the genetic code.
Although MIPs have immense promise for accurately genotyping multi-allelic CNPs such as CCL3L1, it is noteworthy that MIP technology is heavily reliant on high quality reference sequences and availability of singly unique nucleotides (SUNs) to accurately distinguish gene family paralogs.
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