The advantage of using higher biomass concentration has also been studied by other researchers (Saratale et al. 2006; Jadhav and Govindwar 2006; Shedbalkar et al. 2008; Parshetti et al. 2011; Chen and Ting 2015), attributed to availability of more number of cells to secrete extracellular enzymes responsible for biodegradation (Abedin 2008).
Under hypoxic conditions, the hypoxia-inducible factor-1 (HIF-1) activates a large battery of genes involved in increasing O2 availability or in the metabolic adaptation of cells to O2 deprivation within their microenvironment.
We found that a cell input number of 200,000 cells/well provided a good balance between limited availability of cells due to blood volume restrictions and ability to detect all cytokine secretion patterns.
The number of samples included in each assay varied based on the availability of cells at the time of testing.
For the pharmacological assays, each inhibitor was tested on five control cell lines, and the cell lines used for these assays varied based only on the availability of cells at the time of testing.
We took advantage of the availability of these cells to knockdown Rac1 by using siRNAs targeting the human Rac1 gene (Figure 5A-top).
The large amount of thymocytes in the lattice can be explained by the absence of large clusters of the epithelial network, which increase the availability of epithelial cells to interact with thymocytes and decrease the apoptosis by lack of stimulus.
Primary cells have limited proliferative capacity and can be difficult to obtain; therefore, the availability of immortal cells to perform genetic and molecular studies is extremely useful.
In January 2010, border officials stopped processing – fingerprinting and photographing – illegal immigrants caught at Calais due to problems with the availability of cells in which to hold them.
