Species were selected according to their unique evolutionary positions (four mammals, four non-mammal vertebrates and four invertebrates) and sequence availability in the RefSeq database [ 39].
In contrast, MIT-NGS generates genotypes from full-length sequences encompassing all exons, based on allele sequence availability in the IMGT/HLA database.
These variables were selected before analysis by consideration of the potential impact on calcitonin levels, based on an extensive review of the literature and availability in the LEADER database.
The availability in the ESCH database of a group of subjects genotyped for GSTM1 and GSTT1 genes in three national laboratories allowed us to address the question if these genetic polymorphisms can modify the strength of the association between CA and cancer risk.
Based on their availability in the protein database and their diversity, we selected 18 transposases (Table 1), covering at least 8 subfamilies, and used them as queries in a batch TBLASTN search performed on the WGS sequences of 20 Drosophila genomes [ 28] and Piano, Cherbas [ 29], using the default cut-off value.
In two cases (NOZZLE and NAC, substituting NAM), custom-HMMs were used despite their availability in the PFAM databases.
Inclusion criteria for the study were as follows: a complete follow-up at 1 year with available radiographs of the operated hip, no prophylaxis of HO given and the availability of data (in the database or patient case notes or combined).
In addition, an application programme that will check the existence and availability of work operations in the database has to be added to the DBMS.
To thoroughly evaluate the search performance of a database, it is first necessary to consider the 'content coverage', which quantifies the availability of relevant literature contained in the database.
