Crucial for interpretation of microarray data is the availability of a reference to compare signal intensities to reliably determine presence or divergence each DNA fragment.
Besides, it contains attributes related to availability, start time, period, and a reference to its operator.
Ab initio, mapping-first approaches rely on the availability of a reference genome to which the short reads can be aligned.
However, limitations on the availability of a reference genome restricted the amount of information obtained, and phylogenetic distances between these insects may influence the response mechanisms to Cry toxins.
Due to lack of availability of a reference sequence, the assembled transcripts were assumed to be the reference sequence to compute transcript expression levels [ 20, 22, 23].
Finally, we compare the genome sequence assembled using our hybrid strategy to a classical reference assembly using the same data as input and show that with the availability of a reference genome, it pays off to use the hybrid de novo strategy, rather than a classical reference assembly, because more genome sequences are preserved using the former.
A major limitation of this approach, however, is the availability of a reference rock site that is close enough to the soil site to cancel the source and propagation path effects (e.g., Field et al. 1997).
The availability of a reference genome allows the reconstruction procedure to be validated and highlights the diversity between the two genomes.
According to the availability of a reference image, objective IQA indices can be classified as full reference (FR), no-reference (NR), and reduced-reference (RR) methods.
Often, this step is based on the availability of a reference database of images of known objects to perform object recognition or pattern matching.
However, this situation requires the availability of a reference method or data sets, in order to count true and false positives, as well as true and false negatives.
