The inability to establish stable episomal replication in ORC2-hypomorphic cells suggests that the maintenance of both genomes depends on either one or few inefficient origins per episome and that the intracellular concentration of ORC proteins is crucial for the efficient replication of mini-EBV episomes regardless of the origins being used (oriP or auxiliary origins).
This observation suggests the existence of a hierarchical order of strong dominant origins and, of an abundant number of rarely used weak auxiliary origins of DNA replication [39].
Auxiliary origins are not very active in mini-EBVs containing DS (Figs. 5 and 6).
The presence of auxiliary origins in the EBV genome is consistent with the observation that in Raji and other Burkitt's lymphoma cell lines the binding of pre-RC components is also not restricted to oriP [27], [28].
Mungi, C. V. & Rajamani, S. Characterization of RNA-like oligomers from lipid-assisted nonenzymatic synthesis: implications for origin of informational molecules on rarly earth.
Reconstructing early evolutionary events like the origins of informational and operational genes, membranes, and photophosphorylation is difficult because early evolutionary events can be masked by subsequent gene flows.
Yutin et al. (2008) examined individual phylogenies and obtained conflicting results with respect to the euryarchaeal, crenarchaeal, or ancestral archaebacterial origin of eukaryotic informational genes.
They did not state specifically if their target-auxiliary variable relationships passed through the origin and the advantages they found with model-assisted estimation may reflect that issue.
At some stage in the origin of life, an informational polymer must have arisen by purely chemical means.
"Imperfect replication of informational molecules would have marked the origin of both life and evolution, and thus the transition from non-living chemistry to biochemistry," says Bada.
At some point in the origin of life, an informational polymer is believed to have self-assembled by a predisposed chemical route.
