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The plates were digitally scanned and automatically counted with software developed in our group.
BrdU+ cells were automatically counted with a dedicated stereological computer program [49].
The total number of landmark spheres placed in the central brain was automatically counted with Amira (Visage Software, San Diego, CA).
Spots were automatically counted with an electronic computer-assisted imaging system (Autoimmun Diagnostika GmbH, Strassberg, Germany), which has been shown to be valid and precise [ 16].
NCI-H520− and LA-4−transfected clones were selected with 2.0 and 2.5 µg/mL blasticidin (Invitrogen), respectively, for 2 weeks, methanol-fixed, stained with 10% Giemsa, and automatically counted with the Quantity One software (Bio-Rad Laboratories, HerCAles, CA) or UVIDoc software (UVItec Limited, Cambridge, UK).
For a minimal detection of Ca2+ sparks, the criteria was set at greater than 3.8× the standard deviation (SD) of the background noise over the mean background noise, and Ca2+ sparks were automatically counted with the Sparkmaster Plug-in for Image [ 13].
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Preserved zooplankton samples were automatically counted and sized with a bench top model Optical Plankton Counter (Focal Technologies, Dartmouth, Nova Scotia, Canada).
I set up a mix of trackers that automatically counted things like the frequency with which I updated Twitter and uploaded photos to Facebook, and manual ones where I entered the distance I biked, my stress level, alcohol consumption, and more.
Each 24 h, cells were fixed, stained with Hoechst and automatically counted under a fluorescent microscope.
A microscopic image from one preparation magnified 200 times was transferred to a computer program, which automatically counted all positively stained vessels from three random fields of view with the highest density.
Bright punctate EEA1 or LAMP1 staining areas with a diameter of 3 microns or less were automatically counted using the spot count feature for EEA1+LAMP1 +CypHer+ and EEA1+LAMP1+CypHer- cells.
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