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(synthesized by MWG Automatic synthesizer, Germany), 2.5 unit of Taq DNA polymerase (Promega, USA), 2.5 μl of 10 × PCR reaction buffer, 3 mM of MgCl2, 2 μl of DNA template containing 0.5 ng of extracted DNA and total volume rounded to 25 μl by double distilled water.
Peptides consisting of the sequences of the identified and selected epitopes from the screening of the CRA and FRA peptide libraries were synthesized by solid phase using the F-moc strategy and an automatic synthesizer (PSS-8-Shimadzu, Japan).
All peptide conjugates were synthesized according to the standard solid-phase techniques with Fmoc/tBu protocols using a Syro I MultiSynThec GmbH (Wullener, Germany) automatic synthesizer.
PP17 Easy and high yielding synthesis of 68Ga-labelled HBED-PSMA and DOTA-PSMA by using a Modular-Lab Eazy automatic synthesizer.
Peptides synthesis with arginine in position 14 was made on automatic synthesizer using Fmoc strategies, starting with FmocArg(Pbf)NovasynTGA subst (0.22 meq/g) from Novabiochem.
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Using automatic synthesizers, micrograms to kilograms of a specific oligonucleotide can be produced in a few hours.
Two peptide substrates, FKKQGSFAKKK and FKKQGAFAKKK, each with a methacryloyl group at the amino-terminus, were synthesized using an automatic peptide synthesizer according to standard Fmoc-chemistry procedures.
The 66-mer and truncated peptides including 50-, 45-, and 40-mers were synthesized on an automatic peptide synthesizer (C S Bio. Co., USA).
The 11-residue substrate peptide of amino acid sequence His-Lys-Ala-Arg-Val-Leu-NPhe-Glu-Ala-Nle-Ser was synthesized at the National Institute for Research in Reproductive Health, Parel, Mumbai, by using an automatic peptide synthesizer.
Thus, we synthesized the designed molecule on the solid phase considering the future generation of combinatorial libraries using an automatic peptide synthesizer.
The solid-phase synthesis of the peptides, including the peptides for the plaque reduction assay (Table 1), was carried out on a Pioneer automatic peptide synthesizer (Applied Biosystems, Foster City, USA) employing Fmoc chemistry with TBTU/diisopropylethyl amine activation.
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