All fixed testes were embedded in paraffin wax using standard methods in an automatic processor (Leica Microsystems, Milton Keynes, UK) and sections of 5 µm thickness were prepared.
As previously described [24], the NODAGA-RGDyK was radiolabelled with the 68Ga eluate of a 68Ge-generator IGC100 (Eckert & Ziegler, Germany) using an automatic processor unit, Modular-Lab PharmTracer (Eckert & Ziegler, Germany).
Briefly, 1 μg genomic DNA in a volume of 100 μl was sheared into fragments of approximately 300 bp in a 96-well plate with an automatic processor(E210 system; Covaris, Inc. Woburn, MA, USA), using the 10% duty cycle setting, with an intensity of 4, and 200 cycles/burst for 120 seconds.
The plates were processed using an X'Ograph Nodark automatic processor using Devalex M (Champion) and fixed using Fixer Plus (Champion).
The film was processed with an automatic processor (Macrotec MX2).
The signal of the pulse wave was received by a probe (TY-306, Fukuda, Tokyo, Japan) placed over the carotid and femoral arteries and connected with the automatic processor.
The results were obtained with a multiwell plate automatic processor (Techno Genetics, Milan, Italy).
ER and PR expression were evaluated in tumor tissue using the DAKO HORIZON automatic processor (Techmate Horizon).
Immunoblots were developed using a film automatic processor (SRX-101; Konica Minolta Medical) and films were scanned with a 600 d.p.i.
Immunoblots were developed using a film automatic processor (SRX-101, Konica Minolta Medical), and films were scanned with at 300-d.p.i.
