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The resulting solution is a flexible and powerful system for performing dynamic high throughput automatic microscopy.
This study shows that cytotoxicity of CNTs can be assessed by formazan bioreduction (MTS assay), impedance measurements (xCELLigence RTCA), and automatic microscopy (Cell-IQ Analyzer) with different sensitivity.
LNCaP-S14 cells were incubated with individual compounds (final concentration varied between ~5 and 35 uM depending on the molecular weights of the compounds) for 18 h, followed by fixation, staining, imaging (automatic microscopy) and analysis as described in Methods.
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These results demonstrate the feasibility of the automatic annotation of microscopy-based images in adult C.elegans.
The residues were analyzed by energy-dispersive spectrometry, scanning electron microscopy, and automatic surface area and pore analyzer.
Bacterial cells were counted by fluorescent staining and combined direct microscopy and automatic image analysis; invertebrates (nematodes, mites, insects, oligochaetes) were sampled and their body size measured individually to allow allometric scaling.
Imaging with confocal microscopy and automatic analysis of γ-H2AX positive cells.
Complete microscopic images of full tissue sections will be automatically obtained for later automatic or manual visual analysis (virtual microscopy), allowing large-scale histologic evaluation with high precision across the complete section.
The colleague points to some limits of such a system, but we have prepared a sequel manuscript also to be sent to F1000research, in which all has been "miniaturized" to the use of 96-well plates and a semi-automatic analysis by confocal microscopy.
We report a novel approach to diffraction phase microscopy (DPM) with automatic pinhole alignment.
The spatial filtering method is a useful technique for the dynamic and automatic analysis in digital holographic microscopy.
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