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In addition, the functionality of the automatic medium exchange, and fixation procedures were confirmed.
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Half-volume medium exchange was performed every 3 days with medium containing fresh cytokines.
For the chase, a quick medium exchange was performed.
CR.pIX cells were infected with A/PR/8/34 without medium exchange, after a partial medium exchange (half of the culture), or after a complete medium exchange.
Cells were washed three times by medium exchange and centrifugation.
After 24 hours the differentiation was induced by medium exchange.
Viruses were harvested 24 and 48 hours after medium exchange.
Non-adherent cells were removed by medium exchange.
Confluency of mESC cultures under three different medium exchange schedules was monitored: no exchanges, one exchange (48 h), and two exchanges (48 and 70 h; Fig. 4A.i).
The inserts are located in medium-containing chambers, connected with a medium supply for intermittent or continuous medium exchange.
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