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The count was made in an automatic colony counter (Countermat, Flash IUL Instruments).
Counting was performed by an automatic colony counter (BioCount, Bio-Sys GmbH, Karben, Germany).
After incubation, colony forming units (CFU) were counted using an improved Image Analyzer Automatic Colony Counter (Protos, Synoptics, Cambridge, UK), as described elsewhere [ 15].
After 2 weeks, colonies were fixed in 70% ethanol and then stained with 0.25% methylene blue and 30% ethanol, and colonies were counted with an automatic colony counter (MiniCount, IPI Inc).
We incubated the plates for 3 days at 37°C, counted the colonies [revertants (rev)] on an automatic colony counter, and calculated linear regressions over the linear portion of the dose response curves to determine the mutagenic potencies (rev/L-eq).
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Colonies were counted with an automated colony counter (Oxford Optronix, Oxford, UK) following MTT assay in order to increase the contrast and allow automatic detection of spheres.
Colonies were counted using an aCOLyte automated colony counter (Synbiosis, Cambridge, England) and expressed as cfu g-1 of diced onions.
Colonies were counted using an aCOLyte automated colony counter (Synbiosis).
Colony counting was performed by ColCount automated colony counter (Oxford Optronix, Milton, UK).
Colonies were counted manually and by a ColCount automated colony counter (Oxford Optronix).
Colonies grown on soft agarose were counted by automated colony counter.
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