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Incorporated radioactivity was quantified by harvesting cells onto glass-fiber filters (Printer Filtermat A; Wallac, Turku, Finland) using an automatic cell harvester (Harvester 96 Mach III; Tomtec Inc, Orange, CT, USA) and then treated with melt-on scintillator sheets (MeltiLex A; Wallac) and counted with a scintillation beta counter (MicroBeta TriLux; Wallac).
Next, cells were harvested with an automatic cell harvester (Pharmacia, Uppsala, Sweden) and radioactivity was measured in a β-plate liquid scintillation counter (Wallac, Turku, Finland).
Cells were pulsed with 1 μCi [H] thymidine during the last 8 hours of culture, harvested onto glass filters with an automatic cell harvester (Perkin Elmer, Courtaboeuf, France), and the uptake of [H] thymidine was monitored using a 1450-Microbeta Jet β-counter (Perkin Elmer).
Cells were pulsed with 18·5 kBq/well of [H]-thymidine ([H]-TdR; Perkin Elmer, Boston, MA, USA) during the last 8 h of culture, harvested onto glass filters with an automatic cell harvester (Cambridge Technology, Cambridge, MA, USA), and counted using the LKB Betaplate scintillation counter (Wallac, Gaithersburg, MD, USA).
The cells were harvested in glass fiber filters with an automatic cell harvester and radioactive incorporation was measured with a liquid scintillation spectrometer.
Radiolabeled nucleotides were precipitated with cold 10% trichloroacetic acid on filter paper plates in an automatic cell harvester and washed with 95% ethanol.
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The cells were washed three times in ice-cold PBS using a semi-automatic cell harvester (model 48LT; Brandel, Gaithersburg, MD, USA) before counting.
Cells were harvested using an automated cell harvester and [H]-thymidine incorporation into DNA was quantified using a scintillant sheet and counter (Meltilex; Wallac, Turku, Finland).
During the last 16 hours of culture, cells were pulsed with 1 μCi [H]thymidine (Amersham, Buckinghamshire, UK) and subsequently harvested using an automated cell harvester (Pharmacia, Uppsala, Sweden).
[methyl-H]-Thymidine (20 μCi/mL) (PerkinElmer, Waltham, MA, USA) was added 24 hours before harvesting cells on fiberglass filters by means of an automated cell harvester (PerkinElmer).
Cells were collected with an automated cell harvester, and the incorporated radioactivity was measured by liquid scintillation spectrometry.
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